VEGF expression is reduced after Src or STAT3 inhibition. A) Canine OSA cell line OSA8 and human OSA cell line U2OS were treated with LLL3 for 72 hours. RNA was collected and RT-PCR was performed for VEGF and GAPDH. B) Canine and human OSA cell lines were treated with DMSO, SU6656, or LLL3 for 72 hours. Cells were collected and protein lysates were separated via SDS-PAGE. Western blotting for VEGF and β-actin was performed.
Fossey et al. BMC Cancer 2009 9:81 doi:10.1186/1471-2407-9-81