Downregulation of Src or STAT3 leads to loss of MMP2 expression in OSA cells. A) Canine OSA cell lines OSA8 and OSA 32 were treated with DMSO or LLL3 (40 uM), for 72 hours. RNA was collected and RT-PCR was performed for MMP2 and GAPDH. B) Canine and human OSA cell lines were treated with DMSO, SU6656, or LLL3 for 72 hours. Media was collected and MMP2 was assessed via gel zymography. C) Gel zymography images were evaluated by densitometry using NIH Image J.
Fossey et al. BMC Cancer 2009 9:81 doi:10.1186/1471-2407-9-81