Research article
Selenium enrichment of broccoli sprout extract increases chemosensitivity and apoptosis of LNCaP prostate cancer cells
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* Corresponding author: Rizky Abdulah abdulahrizky@gmail.com
1 Department of Public Health, Gunma University Graduate School of Medicine, Japan
2 Department of General Surgical Science (Surgery I), Gunma University Graduate School of Medicine, Japan
3 Department of Urology, Gunma University Graduate School of Medicine, Japan
4 Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Japan
5 Faculty of Pharmacy, Padjadjaran University, Indonesia
6 Faculty of Medicine, Padjadjaran University, Indonesia
BMC Cancer 2009, 9:414 doi:10.1186/1471-2407-9-414
Published: 30 November 2009Abstract
Background
Broccoli is a Brassica vegetable that is believed to possess chemopreventive properties. Selenium also shows promise as an anticancer agent. Thus, selenium enrichment of broccoli has the potential to enhance the anticancer properties of broccoli sprouts.
Method
Selenium-enriched broccoli sprouts were prepared using a sodium selenite solution. Their anticancer properties were evaluated in human prostate cancer cell lines and compared with those of a control broccoli sprout extract.
Results
Selenium-enriched broccoli sprouts were superior to normal broccoli sprouts in inhibiting cell proliferation, decreasing prostate-specific antigen secretion, and inducing apoptosis of prostate cancer cells. Furthermore, selenium-enriched broccoli sprouts but, not normal broccoli sprouts, induced a downregulation of the survival Akt/mTOR pathway.
Conclusion
Our results suggest that selenium-enriched broccoli sprouts could potentially be used as an alternative selenium source for prostate cancer prevention and therapy.