Additional file 6.

Knock-down of WNT5A leads to a reduction in colony formation. NT2/D1 cells were infected with lentiviral shRNAs to knockdown WNT5A expression, and results were compared to a control shRNA. Graphs depict a representative experiment performed in triplicate. Error bars represent standard deviation. a) WNT5A expression was measured using a real-time PCR assay. b) The ability of the cells to form colonies when plated at a low density is displayed. (* p < 0.05).

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Snow et al. BMC Cancer 2009 9:383   doi:10.1186/1471-2407-9-383