Clinical relevance of nine transcriptional molecular markers for the diagnosis of head and neck squamous cell carcinoma in tissue and saliva rinse

doi:10.1186/1471-2407-9-370

BMC Cancer

Volume 9

Viewing options:

Abstract
Full text
PDF (839KB)

Associated material:

Related literature:

Articles citing this article
on Google Scholar
on ISI Web of Science
on PubMed Central
Other articles by authors
on Google Scholar

Lallemant B
Evrard A
Combescure C
Chapuis H
Chambon G
Raynal C
Reynaud C
Sabra O
Joubert D
Hollande F
Lallemant JG
Lumbroso S
Brouillet JP

on PubMed

Lallemant B
Evrard A
Combescure C
Chapuis H
Chambon G
Raynal C
Reynaud C
Sabra O
Joubert D
Hollande F
Lallemant JG
Lumbroso S
Brouillet JP
Related articles/pages
on Google

on Google Scholar

on PubMed

Tools:

Nominate for award

Post to:

Research article

Clinical relevance of nine transcriptional molecular markers for the diagnosis of head and neck squamous cell carcinoma in tissue and saliva rinse

Benjamin Lallemant¹^,2^,3^,4 , Alexandre Evrard²^,3^,4^,5 , Christophe Combescure⁶^,7 , Heliette Chapuis⁸ , Guillaume Chambon¹ , Caroline Raynal²^,3^,4^,5 , Christophe Reynaud¹ , Omar Sabra¹ , Dominique Joubert²^,3^,4 , Frédéric Hollande²^,3^,4 , Jean-Gabriel Lallemant¹ , Serge Lumbroso²^,3^,4^,5 and Jean-Paul Brouillet²^,3^,4^,5

¹Service d'ORL et Chirurgie Maxillo-faciale, Centre Hospitalier Universitaire de Nîmes, Place du Pr. Robert Debré, 30029 Nîmes Cedex 9, France

²Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Institut de Génomique Fonctionnelle, Montpellier F-34094, France

³Institut National de la Santé et de la Recherche Médicale, U661 Montpellier F-34094, France

⁴Université Montpellier 1, Montpellier F-34094, France

⁵Laboratoire de Biochimie, Centre Hospitalier Universitaire de Nîmes, Place du Pr. Robert Debré, 30029 Nîmes Cedex 9, France

⁶Service d'Information Médicale et de Biostatistique, Centre Hospitalier Universitaire de Nîmes, Place du Pr. Robert Debré, 30029 Nîmes Cedex 9, France

⁷CRC - Service d'Epidémiologie Clinique, Hôpitaux Universitaires de Genève, Rue Micheli-du-Crest 24, CH-1211 Genève 14, Suisse

⁸Service d'Anatomie et Cyto-pathologie, Centre Hospitalier Universitaire de Nîmes, Place du Pr. Robert Debré, 30029 Nîmes Cedex 9, France

author email corresponding author email

BMC Cancer 2009, 9:370doi:10.1186/1471-2407-9-370


Published:	18 October 2009

Abstract

Background

Analysis of 23 published transcriptome studies allowed us to identify nine genes displaying frequent alterations in HNSCC (FN1, MMP1, PLAU, SPARC, IL1RN, KRT4, KRT13, MAL, and TGM3). We aimed to independently confirm these dysregulations and to identify potential relationships with clinical data for diagnostic, staging and prognostic purposes either at the tissue level or in saliva rinse.

Methods

For a period of two years, we systematically collected tumor tissue, normal matched mucosa and saliva of patients diagnosed with primary untreated HNSCC. Expression levels of the nine genes of interest were measured by RT-qPCR in tumor and healthy matched mucosa from 46 patients. MMP1 expression level was measured by RT-qPCR in the salivary rinse of 51 HNSCC patients and 18 control cases.

Results

Dysregulation of the nine genes was confirmed by the Wilcoxon test. IL1RN, MAL and MMP1 were the most efficient diagnostic markers of HNSCC, with ROC AUC > 0.95 and both sensitivity and specificity above 91%. No clinically relevant correlation was found between gene expression level in tumor and T stage, N stage, tumor grade, global survival or disease-free survival. Our preliminary results suggests that with 100% specificity, MMP1 detection in saliva rinse is potentially useful for non invasive diagnosis of HNSCC of the oral cavity or oropharynx, but technical improvement is needed since sensitivity was only 20%.

Conclusion

IL1RN, MAL and MMP1 are prospective tumor diagnostic markers for HNSCC. MMP1 overexpression is the most promising marker, and its detection could help identify tumor cells in tissue or saliva.