FER, CTNND1, CAV1 and their phosphorylated version expression in two HCC cell lines. First, equal protein lysates from two HCC cells lines were subjected to SDS-PAGE electrophoresis and subsequent Western blotting using anti-FER, CTNND1, CAV1 and actin antibodies (lower panel). Then in parallel experiment, equal protein lysates from two cell lines were immunoprecipitated using anti-pTyr 4G10 and pTyr100 antibodies, followed by reacting with FER, CTNND1 and CAV1 antibodies in Western blot assay (upper panel). This represents three independent experiments.
Li et al. BMC Cancer 2009 9:366 doi:10.1186/1471-2407-9-366