Scheme of enrichment and identification of pTyr proteins. Cells were firstly lysed in modified RIPA buffer. pTyr proteins and their interacting partners were then immunoprecipitated by anti-pTyr antibodies. Eluted proteins were further digested into peptides and used to IMAC for second phosphopeptides enrichment. The sample was lastly analyzed by LC-MS/MS.
Li et al. BMC Cancer 2009 9:366 doi:10.1186/1471-2407-9-366