Figure 4.

Claudin-4 expression in the plasma of ovarian cancer patients. A, Representative examples claudin-4 detection in plasma samples by immunoblotting. N21 to N28 are non-cancer plasma controls. P21 to P30 are plasma from ovarian cancer patients. C is a positive control (BG-1 culture media). B, CA125 levels in plasma of ovarian cancer patients are compared to claudin-4 expression levels. ++, claudin-4 level is greater than 0.15 μl of BG-1 24 h culture media; +, claudin-4 level is greater than 0.07 μl of BG-1 24 h culture media; -, no detectable expression of claudin-4. CA125 is plotted on a log scale and the threshold of 125 U/ml is shown by a dotted line.