Suppression of the endogenous CCND1 by miR-503. A and B, Western blot revealing reduction of CCND1 protein in miR-503 transfected UMSCC10B cells with quantitative measurement of three experiments (B). C, The same miR-503 transfected UMSCC10B cells were subject to RNA extraction and real time PCR analysis. D. Luciferase assays in UMSCC10B cells. Values in B, C and D are means ± SE of three separate experiments. **, p < 0.01. Expt, Experiment.
Jiang et al. BMC Cancer 2009 9:194 doi:10.1186/1471-2407-9-194