Table 1

Primer sequences and annealing temperatures

Target Gene

Primer Sequence

Annealing temperature

Reference

CYP19A1

F: 5'-acccttctgcgtcgtgtca-3'

R: 5'-tctgtggaaatcctgcgtctt-3'

54

[13,15]

CYP19A1 Promoter I.3

F: 5'-gataaggttctatcagacc-3'

R: 5'-caggaatctgccgtgggaga-3'

53

[13,15]

CYP19A1 Promoter I.4

F: 5'-gtgaccaactggagcctg-3'

R: 5'-caggaatctgccgtgggaga-3'

55

[13,15]

CYP19A1 Promoter II

F: 5'-gcaacaggagctatagat-3'

R: 5'-caggaatctgccgtgggaga-3'

54

[13,15]

18S

F: 5'-cggctaccacatccaaggaa-3'

R: 5'-gctggaattaccgcggct-3'

58

ERα

F: 5'-tgtccagccaccaaccagt-3'

R: 5'-tttcaacattctccctcctctt-3'

55

LRH-1

F: 5'-ctgatactggaacttttgaa-3'

R: 5'-cttcatttggtcatcaacctt-3'

55

[10]

CCND1

F: 5'-aactacctggaccgcttcct-3'

R: 5'-ccacttgagcttgttcacca-3'

55

FSHR

F: 5'-gcggaaccccaacatcgtgtc-3'

R: 5'-tgaagaaatctctgcgaaagt-3'

55

[14]

Chand et al. BMC Cancer 2009 9:148   doi:10.1186/1471-2407-9-148