Figure 7.

The effect of histamine and/or TNF-α on the growth of keratinocyte epithelium in a representative 3-day culture by using 10% fetal calf serum and DMEM as the culture medium. The following agents were added to the wells: well 1, diluent control; well 2, 0.1 mM histamine; well 3, 10 ng mL^-1 TNF-α; well 4, 50 ng mL^-1 TNF-α; well 5, 0.1 mM histamine + 10 ng mL^-1 TNF-α; well 6, 0.1 mM histamine + 50 ng mL^-1 TNF-α. Histamine (0.1 mM) or TNF-α (10 or 50 ng/ml) alone inhibits the growth of epithelium and the growth-inhibitory effect is increased when both agents are added to the same well.