Figure 3.

The effect of histamine (1 mM) and/or TNF-α (10 and 50 ng/ml) on the DNA content of normal keratinocytes. The cells were cultivated in incomplete or complete Keratinocyte-SFM medium and treated with indicated concentrations of histamine and/or TNF-α. The results are expressed as the percentage of the fluorescence in the control wells. Each bar represents the mean of four individual quadruplicate cultures ± SE. "*" denotes p < 0.05 when compared with the control values.