(a) : Breast cancer specimen with high vascular endothelial growth factor-C expression. Note the typical cytoplasmatic staining reaction, original magnification 400×. (b) : Breast cancer with high cyclooxygenase-2 expression. Typically granular staining was diffuse in cytoplasm of the cancer cells, original magnification 400×. (c) : D2-40-stained lymphatic vessel (arrows) with tumor cells (T) inside (lymph vessel invasion) was noted, original magnification 200×. (d) : Breast cancer specimen with a high peritumoral lymphatic vessel density (LVD), some of the lymphatic vessels stained for D2-40 are marked with arrows, original magnification 200×. (e-f): Double staining for D2-40 (brown) and Ki-67 (red) of lymph vessels; Positive staining for Ki-67 is seen in nuclei of lymphatic endothelial cells (black arrows), original magnification 400×.
Zhang et al. BMC Cancer 2008 8:4 doi:10.1186/1471-2407-8-4