Figure 3.

DCX and phospho-DCX (P-DCX) expression in SK-N-SH cell line. Lysates (50 μg/lane) of SK-N-SH control and 6 days RA treated cells were processed with or without calf intestine alkaline phosphatase (CIP) and analyzed by WB. DCX is a phosphoprotein and appears as a doublet that resolves to a single band after CIP treatment (+CIP) in both C and RA samples, indicating that the slower-migrating species is phosphorylated DCX (P-DCX).