Table 1

Sequences of oligonucleotide primers used in real-time PCR and sequencing reactions

Primer pairs used in real-time PCR reactions

Gene
Primer sequence

ACTB
Forward: 5'-TGCGTTACACCCTTTCTTGACA

Reverse: 5'-GCAAGGGACTTCCTGTAACAATG
GAPDH
Forward: 5'-GAAGGTGAAGGTCGGAGTC

Reverse: 5'-GAAGATGGTGATGGGATTTC
MT-ATP6
Forward: 5'-TAGCCATACACAACACTAAAGGACGA

Reverse: 5'-GGGCATTTTTAATCTTAGAGCGAAA
MT-CO1
Forward: 5'-GACGTAGACACACGAGCATATTTCA

Reverse: 5'-AGGACATAGTGGAAGTGAGCTACAAC
MT-CYB
Forward: 5'-ATCACTCGAGACGTAAATTATGGCT

Reverse: 5'-TGAACTAGGTCTGTCCCAATGTATG
MT-ND1
Forward: 5'-CCACCTCTAGCCTAGCCGTTTA

Reverse: 5'-GGGTCATGATGGCAGGAGTAAT
MT-ND6
Forward: 5'-CAAACAATGTTCAACCAGTAACCACTAC

Reverse: 5'-ATATACTACAGCGATGGCTATTGAGGA
MT-RNR1
Forward: 5'-TAGAGGAGCCTGTTCTGTAATCGAT

Reverse: 5'-CGACCCTTAAGTTTCATAAGGGCTA
UBC
Forward: 5'-ATTTGGGTCGCGGTTCTTG

Reverse: 5'-TGCCTTGACATTCTCGATGGT


Primers used in sequencing reactions

Primer name
Primer sequence

D1F A
Forward: 5'-AATGGGCCTGTCCTTGTAG
D2F
Forward: 5'-CGACATCTGGTTCCTACTTC
D3F
Forward: 5'-CGCTTCTGGCCACAGCAC
L16112F
Forward: 5'-CACCATGAATATTGTACGGT
D2R
Reverse: 5'-GGGTTTGGTTGGTTCGGG
D3R A
Reverse: 5'-GGTGTGGCTAGGCTAAGC
DLP4R
Reverse: 5'-GTGGAAAGTGGCTGTGCAG
H16220R
Reverse: 5'-TTGATTGCTGTACTTGCTTGTAAG
H16540R
Reverse: 5'-GTGGGCTATTTAGGCTTTATGACCCTG

A Primers used for PCR amplification of sequenced mtDNA fragment.

Bragoszewski et al. BMC Cancer 2008 8:292   doi:10.1186/1471-2407-8-292