Table 1

Main characteristics of the antibodies used in immunohistochemistry.
Protein (Clone)	Antibody	Origin	Clone	Pre-treatment	Dilution	Location of staining	Normal

Caveolin 1	mmb	Santa Cruz	N20	Micro waves (10 min), tps citrate pH = 6	1/1000	cytoplasm	m.e.c+
Caveolin 2	mmb	Transduction Laboratories	65	Target retrival solution (98°C, 40 min)	1/50	cytoplasm	m.e.c+
Cytokeratin 5/6	mmb	Dako	D5/16 B4	Target retrival solution (98°C, 40 min)	1/10	cytoplasm	m.e.c +
Cytokeratin 14	mmb	Newcastle UK	LL002	Target retrival solution (98°C, 40 min)	1/300	Cell membrane	m.e.c+
EGFR	mmb	Zymed	3IG7	Target retrival solution (98°C, 40 min)	1/10	membrane	m.e.c +
ERBB2	mmb	Dako Herceptest Ltd	AO485	Target retrival solution (98°C, 40 min)	1/500	membrane	-
Estrogen receptor (ER)	mmb	Novocastra laboratories Ltd	6F11.2	Target retrival solution (98°C, 40 min)	1/60	nucleus	m.e.c- and luminal+
P53	mmb	Dako	DO-1	Target retrival solution (98°C, 40 min)	1/4	nucleus	-
P-cadherin	mmb	Transduction laboratories	56	Target retrival solution (98°C, 40 min)	1/75	Cell membrane	m.e.c +
Progesterone receptor (PR)	mmb	Dako	PFR 636	Target retrival solution (98°C, 40 min)	1/80	Nucleus	m.e.c- and luminal+
Ki67	mmb	Dako	KI-67	Target retrival solution (98°C, 40 min)	1/100	Nuclear	-
MUC1	mmb	Transgen	H23	none	1/1000	Apical/Cytoplamic	+

mmb: mouse monoclonal antibody m.e.c: myoepithelials cells
Ben Hamida et al. BMC Cancer 2008 8:28 doi:10.1186/1471-2407-8-28