Cigarette smoke induces endoplasmic reticulum stress and the unfolded protein response in normal and malignant human lung cells

Additional file 1:

Supplemental Table S1: Cigarette smoke signature genes. NHBE cells were treated with whole smoke from a reference cigarette and two of the largest-selling commercial brands in the United States. Cigarette smoke "signature genes" (the list of genes showing significant change in expression at 2 h, 4 h, and 24 h post-exposure for all three cigarette treatments) are shown for each time point.

Format: XLS Size: 143KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

Supplemental Table S2: Functional annotation clustering of cigarette smoke signature genes. Cigarette smoke signature genes subjected to functional annotation clustering of Biological Process Gene Ontology categories using the algorithms on the NIH DAVID website. Only significant (mean p value < 0.01) clusters are listed.

Format: XLS Size: 47KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Supplemental Table S3: Suppression of XBP1 splicing by cigarette smoke. Calculation of percent spliced XBP1 RNA. Gel photographs from PCR experiments presented in Figures 8 and 11 were analyzed as described in the Methods section and all gel bands quantified. After normalization, the percent splicing was calculated using the formula: spliced/(unspliced + spliced) × 100.

Format: XLS Size: 27KB Download file

This file can be viewed with: Microsoft Excel Viewer