|
Linear regression analysis to determine which methyl-sensitive restriction enzymes were effective in CpGlobal. |
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| Enzyme |
Theoretical Regression |
Actual Regression |
Adjusted R Square |
Standard Error |
F significance |
|
|
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| AciI |
y = 1.57-1.57x |
y = 0.45-0.46x |
0.86 |
0.06 |
1.11E-09 |
| BstUI |
y = 0.48-0.48x |
y = 0.09-0.11x |
0.87 |
0.01 |
4.39E-10 |
| HinP1I |
y = 0.66-0.66x |
y = 0.95-0.89x |
0.97 |
0.05 |
1.24E-15 |
| HpaII |
y = 1.00-1.00x |
y = 0.94-0.95x |
0.96 |
0.06 |
4.46E-15 |
| HpyCH4IV |
y = 0.44-0.44x |
y = 0.74-0.73x |
0.97 |
0.04 |
7.67E-17 |
|
Theoretical regression formulas were calculated based on the number of recognition sites in lambda DNA for each of the methyl-sensitive restriction enzymes-AciI: 516, BstUI: 157, HinP1I: 215, HpaII and the isoschizomer MspI: 328, and HpyCH4IV: 143. The Actual regression formulae were calculated based on the regression analysis using the theoretical methylation level as a predictor for the results from CpGlobal. | |||||
Anisowicz et al. BMC Cancer 2008 8:222 doi:10.1186/1471-2407-8-222 |
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