Figure 2.

Assessment of CpGlobal using human genomic DNA isolated from whole blood. A) Linearity of CpGlobal for different amounts of lymphocyte DNA. Twofold serial dilutions of genomic DNA from 100 ng down to 3.12 ng digested with HpaII or MspI. Net Luminescence of the no enzyme control is subtracted from the net luminescence of HpaII and MspI and plotted versus concentration of DNA. The points fit with a linear regression (HpaII R² = 0.982 and Msp I R² = 0.999). B) Net HpaII luminescence was divided by net MspI luminescence to get global DNA methylation index (GDMI) and plotted versus DNA concentration.