Evaluation of high-risk Human papillomaviruses type distribution in cervical cancer in Sichuan province of China

En-qi Wu^*¹^,3 , Guo-nan Zhang^*³ , Xiang-hui Yu¹ , Yuan Ren² , Ying Fan³ , Yong-ge Wu¹ , Wei Kong¹ and Xiao Zha²^,3

¹College of Life Science, Jilin University, Changchun 130012, PR China

²Department of Molecular Pharmacology, Sichuan Tumor Hospital, Chengdu 610041, PR China

³Department of Gynecologic Oncology, Sichuan Tumor Hospital, Chengdu 610041, PR China

author email corresponding author email* Contributed equally

BMC Cancer 2008, 8:202doi:10.1186/1471-2407-8-202


Published:	22 July 2008

Abstract

Background

Infection with high-risk human papillomavirus is an important factor associated with cervical cancer, and the distribution of HPV types varies greatly worldwide. Determination of type-specific HPV prevalence constitutes an important step towards the development of vaccines for the prevention of cervical cancer.

Methods

The human papillomavirus (HPV) genotypes in 190 cervical cancer specimens taken from the Sichuan province, the most populous province of Southwest China, were detected by a combination of MY09/11 consensus primers PCR (MY09/11 PCR), type-specific primers one-step PCR (One-step TS PCR) and E6/E7 gene type-specific primers nested PCR (Nested TS PCR). The prevalence and distribution of HPV in patients with cervical cancer, especially for HPV types 16, 18, 52, 58 and 59, suspected to be most common in certain parts of China, was investigated.

Results

The HPV infection rates detected by MY09/11 PCR, One-step TS PCR and Nested TS PCR were 159 (83.7%), 145 (76.3%) and 172 (90.5%), respectively. The overall HPV prevalence was 93.2% (177/190). The positive specimens for HPV16, 18, 52, 58 and 59 detected by One-step TS-PCR were 111 (58.4%), 14 (7.4%), 6 (3.2%), 13 (6.8%) and 4 (2.1%), respectively. By Nested TS-PCR analysis, the detection rates of HPV16, 52, 58 and 59 were increased to 140 (73.7%), 30 (15.8%), 37 (19.5%) and 25 (13.2%), while only 4 (2.1%) additional specimens were found to be infected with HPV18.

Conclusion

Our data demonstrate that, besides HPV 16, which was found to be the most prevalent type, HPV types 58, 52 and 59 are more prevalent than HPV18 in women with cervical cancer in the Sichuan area of China.