Additional file 2.

Real-time RT-PCR confirmation of selected microarray data. Results are for transcripts identified as up- or down-regulated in microarray analysis of cDNA from Ras^V12-, Ras^V12G37-, Ras^V12S35-, Ras^V12C40-, and Rlf-CAAX-infected HME16C. Numbers represent fold expression changes for transcript levels relative to vector-infected pLRT control cells. Parenthetical numbers indicate mean fold expression changes from cDNA microarray analysis (see Additional file above). Sequences of primers used for real-time RT-PCR analysis are as follows: amphiregulin Forward 5'-CGCCGGTGGTGCTGTCGCTCTT-3' Reverse 5'-TCACTCACAGGGGAAATCTCACTC-3', anterior gradient 2 Forward 5'-GGGGTGACCAACTCATCTGGACTCAG-3' Reverse 5'-GACATACTGGCCATCAGGAGAAAGGTGT-3', CD24 Forward 5'-GCTCCTACCCACGCAGATTTATT-3' Reverse 5'-CACGAAGAGACTGGCTGTTGACT-3', c-met Forward 5'-AAATGGCCACGGGACAACACAA-3' Reverse 5'-TGGGCTGGGGTATAACATTCAAGA-3', CTGF Forward 5'-CTGCCCGGGAAATGCTGCGAGGAGT-3' Reverse 5'-CTGCAGGAGGCGTTGTCATTGGTAA-3', CYR61 Forward 5'-CGGCCCAAGTACTGCGGTTCCT-3' Reverse 5'-ATTGGCATGCGGGCAGTTGTAGTT-3', Decorin Forward 5'-ACTTCTGCCCACCTGGACACAACA-3' Reverse 5'-ATGGCAGAGCGCACGTAGACACA-3', DICER-1 Forward 5'-CAGGAAATACCCGTGCAACCAACTA-3' Reverse 5'-GCATTACGGCCATCACAGGACTTC-3', E-cadherin Forward 5'-GGTATCTTCCCCGCCCTGCCAATCC-3' Reverse 5'-AACCGCTTCCTTCATAGTCAAACACGAG-3', EphA2 Forward 5'-CCCCTTCCGCCCCACACTACCTCACAGC-3' Reverse 5'-ACACGGCCCGCATTCCCCAGACTCG-3', Epiregulin Forward 5'-TTGTATTTTTAGTAGAGGCGGGGTTTCA-3' Reverse 5'-TCGGGCACAGATGTTCAAGTCAC-3', ETS-1 Forward 5'-ACTCGGGGGCCAGGACTCTTTTGAA-3' Reverse 5'-CACGGTCCCGCACATAGTCCTTGAA-3', ETS variant gene 5 Forward 5'-TCGGGGACGTCTACGGTTTCTACT-3' Reverse 5'-AAGACTGTAAACGGCTACCATTGA-3', FAT-2 Forward 5'-GCTGGACATCAAACGGGCTAACAT-3' Reverse 5'-ACCGCATCTGAACCCCCACTGAAT-3', HMGA-1 Forward 5'-GCTCACCCTGCCCGCTCCCAACC-3' Reverse 5'-GCCCCAGCCCCTCTTCCCCACAAA-3', HMGA-2 Forward 5'-CTGATAAGCAAGAGTGGGCGGGTGAGAA-3' Reverse 5'-ACAGGGAGTGGGTTGGGGTGGTATTTGA-3', Secreted frizzled-related protein 1 Forward 5'-CCCGCTCCCTTTCCCTCCATA-3' Reverse 5'-GCTCTCACTTTCCGCCCAATCC-3', Tenascin C Forward 5'-GCGGCCCAGAGCGAGGAA-3' Reverse 5'-TATTGCGATGTTGTCACTGGGAGATTTT-3', TGF-a Forward 5'-CCGCCTTGGTGGTGGTCTCC-3' Reverse 5'-AGGGCGCTGGGCTTCTCGTG-3', TGF-β2 Forward 5'-CCCGCCCACTTTCTACAGACCCTACT-3' Reverse 5'-CATTCGCCTTCTGCTCTTGTTTTCAC-3', TGF-β receptor 2 Forward 5'-ACAGTGGCAGTCAAGATCTTTCCCTATG-3' Reverse 5'-CAACTCCGTCTTCCGCTCCTCAG-3', TRIM29 Forward 5'-ATGCATGCGCCACGTTGAGAAGAT-3' Reverse 5'-GGTGAAGCGGCCAGGAGACGAG-3'.

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Oberst et al. BMC Cancer 2008 8:189   doi:10.1186/1471-2407-8-189