Figure 3.

DNA, Western and Immunohistochemical analysis of the TRAIL DR5 C790T mutation. a Chromotagrams showing DR5 C790T in blood, and liver metastases DNA and cDNA. F & R indicate forward and reverse primers respectively, the arrow indicates C790T. b. Western Blot of DR5 for both a wild-type DR5 and the C790T LM. c. Immunohistochemistry for DR5 in matched colon normal (CN), the invasive front (*) of the primary colon tumour (IF), the central region of the primary tumour (CT) and liver metastasis (LM) from GAPDH is shown as a loading control. c both wild-type DR5 and C790T individuals.