Table 1

Analyses of DNA content in control and chrysotile treated HK2 cells, and frequency of apoptosis and multinucleation.

Frequency (%)

DNA index (C)

Max. DNA (C)

DNA index > 5C (%)

Apoptosis (%)

CONTROL

mono

83.40 (563/675)

1.3

7.0

0.5

-

bi

10.97 (74/675)

1.1

4.7

0

-

multi

5.63 (38/675)

0.9

4.3

0

-

1.2

7.0

0.2

0.26 (2/771)

48 h chrysotile + 24 h recovery

mono

54.07 (259/479)

1.6

8.9

3.5

-

bi

24.64 (118/479)

1.4

8.9

2.1

-

multi

21.29 (102/479)

1.2

8.5

3.1

-

1.4

8.9

2.9

4.64 (30/646)

48 h chrysotile +48 h recovery

mono

34.65 (175/505)

1.7

10.2

6.0

-

bi

29.11 (147/505)

1.6

9.8

7.5

-

multi

36.24 (183/505)

1.4

14.5

8.8

-

1.6

14.5

7.4

6.36 (32/503)

48 h chrysotile +72 h recovery

mono

35.57 (180/506)

1.8

17.7

10.8

-

bi

22.73 (115/506)

1.6

14.6

10.0

-

multi

41.70 (211/506)

1.5

14.1

10.0

-

1.6

17.7

10.2

2.58 (13/504)

After chrysotile treatment the cell culture acquired some features, such as increasing of apoptotic, binucleated and multinucleated cells. The software used to quantify the DNA content made possible to verify the percentage of nuclei with DNA content higher than 5C, and to observe the minimum and maximum values of DNA content in all classes analyzed.

Cortez and Machado-Santelli BMC Cancer 2008 8:181   doi:10.1186/1471-2407-8-181