Figure 5.

Scheme of apoptosis, multinucleation and aneuploid HK2 cells formation after chrysotile exposure. After 48 h chrysotile treatment the fibers are internalized and could interact with chromatin when the nuclear envelop breakdown. This interaction could lead to chromatin fragmentation or aneuploid and multinucleation cell formation due to cytokinesis failure. The cytokinesis failure form cells with centrosome amplification, that could entry mitosis with multipolar spindles and complete or not cytokinesis, leading to aneuploid cells and/or multinucleation. Apoptotic cells could be caused by checkpoint activation due several DNA or cell damage, or by centrosome amplification.