Figure 4.

Interphase multinucleate HK2 cell with a phagocytized chrysotile fiber. A) Confocal image of a HK2 cell after 48 h chrysotile exposure showing an internalized fiber (pink) interacting with the nuclei (red, stained with PI). The image stacks were used for 3D reconstruction (B), evidencing the spatial interaction between cell and fiber, where the fiber seems to segment the nucleus.