Figure 2.

DNA Histograms of control and chrysotile treated cells. The nuclear DNA content from control and chrysotile treated cells was quantified independently by image analysis with the software CIRES. In DNA histograms (N vs. C plot) the nuclei of mononucleated cells are represented in black, the nuclei of binucleated cells are in white and the nuclei of multinucleated cells are in gray, and after 48 h chrysotile treatment were used three different times of recovery in free-fiber medium (24 h, 48 h and 72 h).