Chrysotile effects on human lung cell carcinoma in culture: 3-D reconstruction and DNA quantification by image analysis

doi:10.1186/1471-2407-8-181

BMC Cancer

Volume 8

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Research article

Chrysotile effects on human lung cell carcinoma in culture: 3-D reconstruction and DNA quantification by image analysis

Beatriz A Cortez¹^,2 and Glaucia M Machado-Santelli¹

¹Dept. of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Lineu Prestes 1524, 05508-000, Sao Paulo, Brazil

²Dept. of Genetic and Evolutive Biology, Biosciences Institute, University of Sao Paulo, Sao Paulo, Brazil

author email corresponding author email

BMC Cancer 2008, 8:181doi:10.1186/1471-2407-8-181


Published:	27 June 2008

Additional files

Additional file 1:

Schematic representation of laser scanning confocal microscope configuration. A) Fluorescein in green and propidium iodide in red; B) chrysotile in pink.

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Additional file 2:

Chrysotile fibers in an interphasic multinucleated HK2 cell. After 48 h chrysotile exposure and 24 h of recovery in normal medium many fibers were found inside the HK2 cells. In interphasic cells the fibers were normally located in perinuclear region, and also in the middle of the nuclei in multinucleated cells. However, some fibers interacting with the nucleus were observed.

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