Research article
Chrysotile effects on human lung cell carcinoma in culture: 3-D reconstruction and DNA quantification by image analysis
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* Corresponding author: Glaucia M Machado-Santelli gmmsante@usp.br
1 Dept. of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Lineu Prestes 1524, 05508-000, Sao Paulo, Brazil
2 Dept. of Genetic and Evolutive Biology, Biosciences Institute, University of Sao Paulo, Sao Paulo, Brazil
BMC Cancer 2008, 8:181 doi:10.1186/1471-2407-8-181
Published: 27 June 2008Additional files
Additional file 1:
Schematic representation of laser scanning confocal microscope configuration. A) Fluorescein in green and propidium iodide in red; B) chrysotile in pink.
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Additional file 2:
Chrysotile fibers in an interphasic multinucleated HK2 cell. After 48 h chrysotile exposure and 24 h of recovery in normal medium many fibers were found inside the HK2 cells. In interphasic cells the fibers were normally located in perinuclear region, and also in the middle of the nuclei in multinucleated cells. However, some fibers interacting with the nucleus were observed.
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