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Open AccessHighly AccessResearch article

Promoter methylation-associated loss of ID4 expression is a marker of tumour recurrence in human breast cancer

Erik Noetzel1 email, Jürgen Veeck1 email, Dieter Niederacher2 email, Oliver Galm3 email, Felicitas Horn4 email, Arndt Hartmann5 email, Ruth Knüchel1 email and Edgar Dahl1 email

Molecular Oncology Group, Institute of Pathology, University Hospital of the RWTH Aachen, Aachen, Germany

Department of Gynaecology and Obstetrics, Heinrich-Heine University, Düsseldorf, Germany

Medical Clinic IV, University Hospital of the RWTH Aachen, Aachen, Germany

Department of Gynaecology and Obstetrics, University of Regensburg, Regensburg, Germany

Institute of Pathology, University of Erlangen, Erlangen, Germany

author email corresponding author email

BMC Cancer 2008, 8:154doi:10.1186/1471-2407-8-154

Published: 30 May 2008

Additional files

Additional file 1:

"Touchdown PCR for ID4 mRNA expression analysis"

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Additional file 2:

"Positive controls for the immunohistochemical staining with normal and tumourous colon tissues for a polyclonal ID4 antibody (sc-491)." A) and C) are negative controls without ID4 antibody incubation for normal and tumourous colon tissues, respectively. B) Intensive cytoplasmatic staining of a normal colon tissue. D) Negative ID4 protein staining for an infiltrating colon carcinoma (G2, pT2, pN0, pMx).

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