Table 2

Summary of cell line growth characteristics and tumorigenicity

Cell line growth assays

TOV-1946

OV-1946

TOV-2223

TOV-112D


Growth characteristic

Doubling time (days) +/- S.D.a

1.3 +/- 0.4

2.5 +/- 0.9

2.6 +/- 0.7

1.0 +/- 0.2

Saturation density (nb cells +/- S.D.)b

3 053 933 +/- 153 933

3 231 400 +/- 962 723

2 536 867 +/-680 852

6 162133 +/- 515 034

Number of passages to date

>210

>210

>150

>200

Spheroid

Formation

aggregate

semi-compact

no

compact

Migration

time for wound filling (h)

24

48

>48

48

Invasion

Mean number of cells +/- S.D.c

417 +/- 226

149 +/- 70

257 +/- 79

118 +/- 34

Soft agarose

Colony number +/- S.D.d

11 +/- 3

24 +/- 8

16 +/- 4

27 +/- 10

Colony size

large

large

small

large

Low serum

Capacity to grow in low serum (1%) conditions

yes

yes

yes

yes

Subcutaneous injection in SCID mice

Number of mice with tumors (n = 6)

0

0

0

5

Mean time of tumor appearance (days)

N/A

N/A

N/A

7

Intraperitoneal injection in SCID mice

Number of mice with tumors (n = 6)

3

5

0

6

Mean time of tumor appearance (days)

125

63

N/A

18

Number of mice with ascitis

2

1

0

2


N/A not applicable

acalculated according to the slope of the linear portion of the growth curve.

brepresents the mean of maximum number of cells counted among the two independent experiments performed in triplicatat

cnumber of cells that migrate through the matrigel insert

dmean of colony numbers per representative area (minimum 5) from two independent experiment performed in duplicate

Ouellet et al. BMC Cancer 2008 8:152   doi:10.1186/1471-2407-8-152

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