Table 2 |
|||||
|
Summary of cell line growth characteristics and tumorigenicity |
|||||
|
Cell line growth assays |
TOV-1946 |
OV-1946 |
TOV-2223 |
TOV-112D |
|
|
|
|||||
|
Growth characteristic |
Doubling time (days) +/- S.D.a |
1.3 +/- 0.4 |
2.5 +/- 0.9 |
2.6 +/- 0.7 |
1.0 +/- 0.2 |
|
Saturation density (nb cells +/- S.D.)b |
3 053 933 +/- 153 933 |
3 231 400 +/- 962 723 |
2 536 867 +/-680 852 |
6 162133 +/- 515 034 |
|
|
Number of passages to date |
>210 |
>210 |
>150 |
>200 |
|
|
Spheroid |
Formation |
aggregate |
semi-compact |
no |
compact |
|
Migration |
time for wound filling (h) |
24 |
48 |
>48 |
48 |
|
Invasion |
Mean number of cells +/- S.D.c |
417 +/- 226 |
149 +/- 70 |
257 +/- 79 |
118 +/- 34 |
|
Soft agarose |
Colony number +/- S.D.d |
11 +/- 3 |
24 +/- 8 |
16 +/- 4 |
27 +/- 10 |
|
Colony size |
large |
large |
small |
large |
|
|
Low serum |
Capacity to grow in low serum (1%) conditions |
yes |
yes |
yes |
yes |
|
Subcutaneous injection in SCID mice |
Number of mice with tumors (n = 6) |
0 |
0 |
0 |
5 |
|
Mean time of tumor appearance (days) |
N/A |
N/A |
N/A |
7 |
|
|
Intraperitoneal injection in SCID mice |
Number of mice with tumors (n = 6) |
3 |
5 |
0 |
6 |
|
Mean time of tumor appearance (days) |
125 |
63 |
N/A |
18 |
|
|
Number of mice with ascitis |
2 |
1 |
0 |
2 |
|
|
|
|||||
|
N/A not applicable acalculated according to the slope of the linear portion of the growth curve. brepresents the mean of maximum number of cells counted among the two independent experiments performed in triplicatat cnumber of cells that migrate through the matrigel insert dmean of colony numbers per representative area (minimum 5) from two independent experiment performed in duplicate |
|||||
|
Ouellet et al. BMC Cancer 2008 8:152 doi:10.1186/1471-2407-8-152 |
|||||
