Figure 3.

Tumor cell apoptosis after treatment with poly I:C and CHX. (A) FACS analysis of apoptosis in Hela and MCA38 cells after stimulation with poly I:C and CHX. Tumor cells were cultured for 24 h with PBS, poly I:C (100 μg/ml), CHX (1.5 μg/ml) or poly I:C puls CHX, and apoptosis was measured by Annexin V, PI staining. (B) Statistic analysis of apoptotic cells based on Fig 1A. (C) The dynamic analysis of apoptosis of tumor cells. Cancer cells (Hela or MCA38) were cultured with different concentrations of poly I:C (50 or 100 μg/ml) and the same concentration of CHX (1.5 μg/ml) for 12 h or 24 h. PBS was used as control. Apoptosis was measured by flow cytometry with anti-Annexin V and anti-PI antibodies. Data were obtained from three independent experiments.