CD24 cross-linking induces apoptosis in, and inhibits migration of, MCF-7 breast cancer cells

Additional file 2:

Viability of MCF-7 cross-linked with anti-human CD24 mouse monoclonal antibody. A) MCF-7 cells were cross-linked with anti-mouse monoclonal IgG antibody in a dose dependent manner for 72 h. B) MCF-7 cells were cross-linked with 500 ng/ml anti-mouse monoclonal IgG antibody or anti-human CD24 mouse monoclonal antibody for 72 h. A-B) Relative survival cell rate is shown as percent survivals versus in treatment versus control cells where CD24 was cross-linked with anti-mouse monoclonal IgG. Data represent means of at least three independent experiments and standard errors of the means. **, p value of less than 0.01.

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Additional file 3:

Changes in apoptosis after CD24 cross-linking with anti-human CD24 mouse monoclonal antibody. MCF-7 cells were cross-linked with 500 ng/ml anti-mouse monoclonal IgG antibody or anti-human CD24 mouse monoclonal antibody for 72 h. Cells were stained with FITC-conjugated annexin V in a buffer containing propidium iodide and analyzed by flow cytometry. For each group of cells, the percentage of survival is shown in the lower left quadrant, where both in annexin V and propidium iodide levels are low. One of three representative experiments is presented.

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Additional file 1:

CD24 expression and cell viability after CD24 cross-linking in MCF-10A cells. A) CD24 expression was analysed with PE anti-human CD24 antibody by flow cytometry on a FACSCalibur system. One of three the representative experiments are shown in the result. B) MCF-10A was cross-linked with 500 ng/ml anti-rabbit polyclonal IgG or anti-human CD24 rabbit polyclonal antibody 72 h. B) Relative survival cell rate is shown as percent survivals versus in treatment versus control cells where CD24 was cross-linked with anti-rabbit polyclonal IgG. C). MCF-10A was treated with 500 ng/ml of anti-rabbit polyclonal IgG or anti-human CD24 rabbit polyclonal antibody in a time dependent manner for 72 h. C) MCF-10A cell survival is shown versus control cell survival after CD24 cross-linking with anti-rabbit polyclonal IgG. Means of at least three independent experiments are presented with standard errors. *, p value of less than 0.05 **, p value of less than 0.01.

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