Figure 7.

Analysis of BigH3 expression. Analysis of BigH3 expression in pancreatic carcinoma cells retaining endogenous Smad4 expression and upon stable Smad4-knockdown. RNAs from three independent Smad4-positive control clones and three knockdown clones, in which Smad4 expression was reduced to below 10% were hybridised with the BigH3 specific probe.