Figure 1.

Inhibition of proliferation in (A and C) MCF-7 WT or (B and D) MCF-7 ADR cultures with lipoxygenase inhibitors; MK59I, MK886, NDGA for A and B; curcumin, ketoconazole, and AA88I for C and D. Cells were plated in IMEM culture fluid containing 8% FBS, 0.5% penicillin and streptomycin sulfate,0.5% kanamycin sulfate, and 1% MEM Vitamin Solution. Inhibitors were added after 24 hours and the cells were incubated in a humidified chamber of 5% CQ₂ for an additional 48 hours. Thymidine was incorporated 16 hours prior to harvest and the cells were harvested using Packard Unifilter System. Values are expressed as % of the control. Data points represent the mean ± SD of three experiments with samples run in replicates of six.