Table 3

Cycling conditions for the PCRs for the different polymorphisms

Steps

Time

Temperature for the various SNPs


IL1A +4845

IL1B -511

IL1B +3954

IL1RN +2018

IL4R +1902

IL6 -174

IL10 -1082


1

2 minutes

50°C

50°C

50°C

50°C

50°C

50°C

50°C

2

10 minutes

95°C

95°C

95°C

95°C

95°C

95°C

95°C

3

15 seconds

95°C

95°C

95°C

95°C

95°C

95°C

95°C

4

1 minutes

59°C

59°C

61°C

64°C

61°C

62°C

62°C

5

40 times

Go to step 3

Go to step 3

Go to step 3

Go to step 3

Go to step 3

Go to step 3

Go to step 3

6

Hold

15°C

15°C

15°C

15°C

15°C

15°C

15°C


Balasubramanian et al. BMC Cancer 2006 6:188   doi:10.1186/1471-2407-6-188

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