Table 2

Final concentration of the different constituents of the PCR mixture

PCR constituents

Final concentrations for the various SNPs


IL1A +4845

IL1B -511

IL1B +3954

IL1RN +2018

IL4R +1902

IL6 -174

IL10 -1082


Taqman mastermix (2X)

1X

1X

1X

1X

1X

1X

1X

Forward primer (10 μM)

500 nM

100 nM

300 nM

250 nM

50 nM

50 nM

50 nM

Reverse primer (10 μM)

500 nM

100 nM

300 nM

250 nM

500 nM

50 nM

300 nM

FAM probe (5 μM)

50 nM

50 nM

50 nM

30 nM

30 nM

30 nM

50 nM

TET probe (5 μM)

100 nM

100 nM

75 nM

150 nM

120 nM

60 nM

150 nM

Template (20 ng/μl)

0.8 ng/μl

0.8 ng/μl

0.8 ng/μl

0.8 ng/μl

0.8 ng/μl

0.8 ng/μl

0.8 ng/μl


Taqman mastermix: Universal PCR mastermix (PE Biosystems) containing MgCl2, dNTPs, Taq polymerase, optimised buffer components and Rox reference dye; FAM probe: 6-carboxy-fluorescein-labelled probe; TET: 6-carboxy-4,7,2',7'-tetrechlorofluorescein-labelled probe.

Balasubramanian et al. BMC Cancer 2006 6:188   doi:10.1186/1471-2407-6-188

Open Data