Table 2

Running methods and temperature used for DHPLC mutation detection

Gene-Exon (bp)

Starting Gradient (%B)

Temperature (°C)


p53-4

58.2

60.9/62.5

p53-5I

50

64.4

p53-5II

52.9

65.1

p53-6

53.1

61.5

p53-7

53.5

61.7/63.4/64.3

p53-8

54.6

59.8/62.8

p53-9

50.5

58.6/59.6


c-kit-9

56.5

55.3/58.5/59.5

c-kit-11

51.2

57.7/58.7

c-kit-13

55.4

56.7/58.7/59.3

c-kit-17

54.2

56.5/58


EGFR-18 (400)

57

60.9/62.9

EGFR-19 (372)

56.5

60.1

EGFR-20 (409)

57.2

61.2/63.4

EGFR-21 (415)

57.3

60.8


Ambrosini-Spaltro et al. BMC Cancer 2006 6:185   doi:10.1186/1471-2407-6-185

Open Data