Research article
Mutations of PIK3CA in gastric adenocarcinoma
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* Corresponding author: Suet Y Leung suetyi@hkucc.hku.hk
1 Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Hong Kong
2 Department of Surgery, The University of Hong Kong, Queen Mary Hospital, Hong Kong
3 Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA
4 Department of Biopharmaceutical Sciences, University of California, San Francisco, USA
BMC Cancer 2005, 5:29 doi:10.1186/1471-2407-5-29
Published: 23 March 2005Additional files
Additional File 1:
Primers and PCR conditions used for PIK3CA sequencing. Conditions for PCR and sequences of primers used in PIK3CA sequencing
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Additional File 2:
SAM significant genes list associated with PIK3CA expression. SAM detected 2910 genes whose expression levels were positively associated with the PIK3CA expression level. Gene expression data were extracted from the microarray database containing 126 samples (90 gastric adenocarcinomas, 14 lymph node metastasis and 22 non-tumour gastric mucosae) based on a 3-fold signal above background ratio for either channel and with 80% good data [14]. Gene expression data from 20,336 cDNA clones satisfied this selection criteria and were extracted, which included a cDNA clone corresponding to PIK3CA (IMAGE clone number 345430, GenBank accession no. W72473). Expression value of this clone was imputed as a quantitative variable for SAM analysis. The missing values in the dataset were estimated by a K-nearest neighbours impute algorithm using 10 nearest neighbour followed by 5000 permutations in the SAM analysis.
Format: XLS Size: 729KB Download file
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