Figure 1.

Restriction map of plasmid pMOhly-Raf The plasmid pMOhly-Raf contains the intact structural genes hlyC, hlyB and hlyD of the hemolysin operon and the c-raf-hlyA fusion hybrid gene. All genes are transcribed from the original cis-acting expression sites in front of hlyC [27]. Abbreviations: ori – origin of replication, bla – ampicillin resistance cassette