Table 1

Secreted cytokine array. Secreted proinflammatory cytokines down-regulated by HA, anti-MIF antibody and MIF anti-sense. Integrated density values (IDV, average intensity times total area) were determined for each individual cytokine dot. Individual cytokine dot intensities were normalized to HRP-positive controls on each membrane. Mean control data for each cytokine spot are expressed as protein ratios (cytokine specific band integrated density value divided by HRP-positive control integrated density value). Treatment data are expressed as +, greater than 2 fold increase in secretion compared with control cytokine spot; -, greater than 2 fold decrease in secretion compared with control cytokine spot; (-), decreased beyond detection.

Cytokine Family


Control (Mean Protein Ratio)

HA

α-MIF

AS


Chemokine CC

CCL2

1.27 ± 0.04

-

(-)

CCL4

0.30 ± 0.02

-

(-)

(-)

CCL5

0.18 ± 0.01

-

(-)

(-)

CCL11

0.11 ± 0.01

(-)

(-)

(-)

CCL15

0.41 ± 0.04

(-)

(-)

-

CCL17

0.12 ± 0.01

-

(-)

(-)

CCL18

1.97 ± 0.07

-

(-)

-

CCL20

0.69 ± 0.04

(-)

-

CCL22

0.19 ± 0.02

-

(-)

-

CCL23

0.12 ± 0.01

(-)

(-)

(-)

CCL24

0.14 ± 0.01

-

(-)

-

CCL26

0.20 ± 0.01

(-)

(-)

(-)

CXC Chemokine

CXCL1

0.16 ± 0.02

-

-

(-)

CXCL2

0.15 ± 0.02

-

-

(-)

CXCL5

0.19 ± 0.02

-

(-)

(-)

CXCL6

0.26 ± 0.03

-

-

-

CXCL7

1.56 ± 0.03

-

(-)

-

CXCL8

0.32 ± 0.02

-

-

CXCL9

0.16 ± 0.01

-

(-)

-

CXCl10

1.60 ± 0.17

-

-

-

CXCL12

0.15 ± 0.01

-

(-)

-

CXCL13

0.34 ± 0.02

-

(-)

-

CXC3C Chemokine

CX3CL1

0.19 ± 0.01

-

(-)

Cytokines

Leptin

0.19 ± 0.02

-

(-)

(-)

LIF

3.20 ± 0.04

-

MIF

0.64 ± 0.04

(-)

(-)

(-)

OSM

0.20 ± 0.02

-

(-)

(-)

Interferon

INF-γ

0.17 ± 0.02

-

(-)

(-)

Neurotropins

BDNF

1.10 ± 0.05

-

-

GDNF

1.30 ± 0.07

-

NT-3

2.00 ± 0.11

-

(-)

NT-4

0.79 ± 0.04

-

(-)

-

Enzymes

TIMP-1

2.54 ± 0.11

-

TIMP-2

5.29 ± 0.29

-

+

Growth Factors

Ang-1

0.20 ± 0.02

(-)

(-)

EGF

0.17 ± 0.03

-

(-)

-

FGF-4

0.24 ± 0.03

-

(-)

(-)

FGF-6

0.18 ± 0.01

-

(-)

-

FGF-9

0.81 ± 0.05

-

HGF

0.12 ± 0.02

-

(-)

(-)

MCSF

0.15 ± 0.02

-

(-)

(-)

PGIF

0.64 ± 0.05

-

(-)

-

Inhibin

0.17 ± 0.02

-

(-)

(-)

TGF β-2

3.20 ± 0.03

-

TGF β-3

0.77 ± 0.02

-

(-)

(-)

Hormone-like protein

IGF-1

0.14 ± 0.01

(-)

(-)

(-)

IGFBP-1

2.40 ± 0.10

-

IGFBP-2

1.40 ± 0.06

-

-

IGFBP-3

2.50 ± 0.12

-

(-)

IGFBP-4

0.11 ± 0.01

-

(-)

-

TP-3

0.13 ± 0.01

-

(-)

(-)

Interleukins

IL-1α

0.20 ± 0.01

-

(-)

(-)

IL-1β

0.14 ± 0.02

-

(-)

(-)

IL-2

0.14 ± 0.04

-

(-)

(-)

IL-12

0.16 ± 0.01

-

(-)

(-)

IL-15

0.10 ± 0.09

-

(-)

(-)

IL-16

0.27 ± 0.04

-

(-)

-

PDGF Superfamily

PDGF-B

0.17 ± 0.02

-

(-)

(-)

P1GF

0.59 ± 0.04

-

(-)

-

VEGF

3.20 ± 0.48

-

+

-

TNF Superfamily

TNFSF14

0.19 ± 0.01

-

-

(-)

TNFSF2

0.13 ± 0.02

(-)

(-)

-

TNFRSF11B

4.40 ± 0.04

-

CD Ligands

Fit-3

0.07 ± 0.01

-

-

(-)


Meyer-Siegler et al. BMC Cancer 2004 4:34   doi:10.1186/1471-2407-4-34

Open Data