Table 1 |
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|
RT-PCR primer pairs used for mesothelin transcript amplification |
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|
amplification target |
sequence, 5' – 3' |
location |
expected product size |
|
|
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|
full length (tr1 & 2) |
ACCCACGGTGCCTCCCTCCC TCAGGCCAGGGTGGAGGCTAG |
exon 1 exon 17 |
1906 bp (tr1) 1924 bp (tr 2) |
|
24 bp insert region (tr1 & 2) |
CTACCTCTTCCTCAAGATGAG GTGTCTAGGGTGTCTTTGTCT |
exon 12 exon 13 |
177 bp (tr1) 201 bp (tr2) |
|
82 bp insert region (tr1 & 3) |
ATGAAGCTGCGGACGGATGCG TGAGAACAGGTCCAGGTCCTA |
exon 15 exon 17 |
262 bp (tr 1) 344 bp (tr 3) |
|
tr3-specific |
ACCCACGGTGCCTCCCTCCC ACCTCCACGCCCCCAGCTCTG |
exon 1 intron 16 |
1873 bp |
|
|
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|
Muminova et al. BMC Cancer 2004 4:19 doi:10.1186/1471-2407-4-19 |
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