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Open Access Research article

Clinical implications in the shift of syndecan-1 expression from the cell membrane to the cytoplasm in bladder cancer

Makito Miyake1, Adrienne Lawton2, Yunfeng Dai3, Myron Chang3, Lourdes Mengual4, Antonio Alcaraz4, Steve Goodison156 and Charles J Rosser157*

Author Affiliations

1 Cancer Research Institute, Orlando Health, Orlando, FL 32827, USA

2 Department of Pathology, Orlando Health, Orlando, FL 32806, USA

3 Department of Biostatistics, The University of Florida, Gainesville, FL 32610, USA

4 Laboratory and Department of Urology, Hospital Clínic, Universitat de Barcelona, Barcelona, Spain

5 Nonagen Bioscience Corp, Orlando, FL 32827, USA

6 Department of Health Sciences Research, Mayo Clinic, Jacksonville, FL 32224, USA

7 University of Hawaii Cancer Center, Clinical and Translational Research Program, 701 Ilalo Street, Honolulu, HI 96813, USA

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BMC Cancer 2014, 14:86  doi:10.1186/1471-2407-14-86

Published: 13 February 2014

Abstract

Background

To determine the diagnostic and prognostic capability of urinary and tumoral syndecan-1 (SDC-1) levels in patients with cancer of the urinary bladder.

Methods

SDC-1 levels were quantitated by enzyme-linked immunosorbent assay (ELISA) in 308 subjects (102 cancer subjects and 206 non-cancer subjects) to assess its diagnostic capabilities in voided urine. The performance of SDC-1 was evaluated using the area under the curve of a receiver operating characteristic curve. In addition, immunohistochemical (IHC) staining assessed SDC-1 protein expression in 193 bladder specimens (185 cancer subjects and 8 non-cancer subjects). Outcomes were correlated to SDC-1 levels.

Results

Mean urinary levels of SDC-1 did not differ between the cancer subjects and the non-cancer subjects, however, the mean urinary levels of SDC-1 were reduced in high-grade compared to low-grade disease (p < 0.0001), and in muscle invasive bladder cancer (MIBC) compared to non-muscle invasive bladder cancer (NMIBC) (p = 0.005). Correspondingly, preliminary data note a shift from a membranous cellular localization of SDC-1 in normal tissue, low-grade tumors and NMIBC, to a distinctly cytoplasmic localization in high-grade tumors and MIBC was observed in tissue specimens.

Conclusion

Alone urinary SDC-1 may not be a diagnostic biomarker for bladder cancer, but its urinary levels and cellular localization were associated with the differentiation status of patients with bladder tumors. Further studies are warranted to define the potential role for SDC-1 in bladder cancer progression.

Keywords:
Syndecan; Bladder; Cancer biomarker; Specificity