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Open Access Research article

MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma

Zhao Sun1, Changting Meng1, Shihua Wang2, Na Zhou1, Mei Guan1, Chunmei Bai1, Shan Lu3, Qin Han2* and Robert Chunhua Zhao24*

Author Affiliations

1 Department of Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China

2 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, People’s Republic of China

3 China National Center for Biotechnology Development, Beijing, People’s Republic of China

4 Center of Translational medicine, Peking Union Medical College Hospital, Beijing, People’s Republic of China

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BMC Cancer 2014, 14:616  doi:10.1186/1471-2407-14-616

Published: 27 August 2014

Abstract

Background

The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.

Methods

Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis.

Results

Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3′-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients.

Conclusions

Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

Keywords:
Hepatocellular carcinoma; Invasion; MicroRNA-1246; CADM1