KIF2A silencing inhibits the proliferation and migration of breast cancer cells and correlates with unfavorable prognosis in breast cancer
1 Department of Pathology and Pathophysiology, School of Medicine, Shandong University, 44# Wen Hua Xi Road, Jinan, Shandong, China
2 School of Stomatology, Shandong University, Jinan, Shandong, China
3 Department of Breast Surgery, Qilu Hospital, Shandong University, 107# Wen Hua Xi Road, Jinan, Shandong, China
4 Institute of Basic Medical Science, Qilu Hospital, Shandong University, Jinan, Shandong, China
5 Shandong Center for Disease Control and Prevention, Jinan, Shandong, China
6 Institute for Global Food Security, School of Biological Sciences, Queen’s University of Belfast, Belfast, UK
BMC Cancer 2014, 14:461 doi:10.1186/1471-2407-14-461Published: 21 June 2014
Kinesin family member 2a (KIF2A), a type of motor protein found in eukaryotic cells, is associated with development and progression of various human cancers. The role of KIF2A during breast cancer tumorigenesis and progression was studied.
Immunohistochemical staining, real time RT-PCR and western blot were used to examine the expression of KIF2A in cancer tissues and adjacent normal tissues from breast cancer patients. Patients’ survival in relation to KIF2A expression was estimated using the Kaplan–Meier survival and multivariate analysis. Breast cancer cell line, MDA-MB-231 was used to study the proliferation, migration and invasion of cells following KIF2A-siRNA transfection.
The expression of KIF2A in cancer tissues was higher than that in normal adjacent tissues from the same patient (P < 0.05). KIF2A expression in cancer tissue with lymph node metastasis and HER2 positive cancer were higher than that in cancer tissue without (P < 0.05). A negative correlation was found between KIF2A expression levels in breast cancer and the survival time of breast cancer patients (P < 0.05). In addition, multivariate analysis indicated that KIF2A was an independent prognostic for outcome in breast cancer (OR: 16.55, 95% CI: 2.216-123.631, P = 0.006). The proliferation, migration and invasion of cancer cells in vitro were suppressed by KIF2A gene silencing (P < 0.05).
KIF2A may play an important role in breast cancer progression and is potentially a novel predictive and prognostic marker for breast cancer.