Additional file 1: Figure S1.
mRNA expression of CSC markers in control and paclitaxel treated ascites-derived tumor cells. RNA from the control and matching paclitaxel treated ascites-derived tumour cells was extracted cDNA was prepared and q-PCR for EpCAM, NANOG CD44, CD117, Oct4, JAGGED, STAT3 and E-cadherin was performed as described in the Methods. The resultant mRNA levels were normalized to 18S mRNA. The experiments were performed using five independent patient samples; the resulting mRNA results were then pooled for analysis. Significant variation is indicated by *P < 0.05.
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Abubaker et al. BMC Cancer 2014 14:317 doi:10.1186/1471-2407-14-317