Stage and tissue-specific prognostic impact of miR-182 in NSCLC
1 Institute of Clinical Medicine, University of Tromso, Tromso, Norway
2 Department of Oncology, University Hospital of North Norway, Tromso 9038, Norway
3 Institute of Medical Biology, University of Tromso, Tromso, Norway
4 Department of Clinical Pathology, University Hospital of North Norway, Tromso, Norway
BMC Cancer 2014, 14:138 doi:10.1186/1471-2407-14-138Published: 27 February 2014
MicroRNA (miR)-182 is frequently upregulated in cancers, has generally been viewed as an oncogene and is possibly connected to angiogenesis. We aimed to explore what impact miR-182 has in non-small cell lung cancer (NSCLC), and more explicitly its correlation with angiogenic markers.
From 335 unselected stage I to IIIA NSCLC carcinomas, duplicate tumor and tumor-associated stromal cores were collected in tissue microarray blocks (TMAs). In situ hybridization (ISH) was used to detect the expression of miR-182 in tumor cells, and immunohistochemistry (IHC) was used to detect the expression of angiogenesis related protein markers.
In univariate analyses, high tumor cell expression of miR-182 was a positive prognostic factor for patients with squamous cell carcinoma (SCC, P = 0.042) and stage II patients (P = 0.003). Also in the multivariate analysis, high tumor cell miR-182 expression was associated with a good prognosis in the same groups (SCC: HR 0.57, CI 95% 0.33-0.99, P = 0.048; stage II: HR 0.50, CI 95% 0.28-0.90, P = 0.020). We found significant correlations between miR-182 and the angiogenesis related markers FGF2, HIF2α and MMP-7.
In patients with SCC and in stage II patients, high tumor cell miR-182 expression is an independent positive prognostic factor.