Both Th1 and Th2 cytokines are secreted by armed ATC. Unarmed or EGFRBi-armed ATC (107) were incubated overnight alone or with U251MG cells (E:T = 25:1). The values are reported as pg/106/24 hours in 1 ml cultures. The culture supernatant was assayed by Bio-Plex assay for the presence of Th1 and Th2 cytokines. Data shown are for each of the ATC donors individually; the horizontal line indicates the median value. The figure shows the concentrations only for those cytokines which showed differences between effectors alone and effectors plus targets.
Zitron et al. BMC Cancer 2013 13:83 doi:10.1186/1471-2407-13-83