BIRC5 promoter activity in HCC cells. (A) Scheme of binding sites for transcription factors in the BIRC5 proximal promoter region. (B) Cell lines, including the indicated parental, adenovirus-infected and shRNA-transfected cells, were seeded on 24-well plates at a density of 5 × 104 cells/well and transfected with the BIRC5 promoter-driven luciferase plasmid pSRVN-Luc (200 ng/well). The relative activity of BIRC5 promoter in HCC cells was measured and shown in histograms; *, P < 0.05; **, P < 0.01. (C) Cells were seeded on 96-well plates at a density of 5 × 103 cells/well, transfected with the BIRC5 promoter-driven EGFP plasmid pSRVN-EGFP (2 μg/well), and observed under a fluorescent microscope 48 h later, original magnification 200 × .
Cao et al. BMC Cancer 2013 13:82 doi:10.1186/1471-2407-13-82