Sub-cytotoxic MJ suppressed the expression of VEGF in gastric cancer cells. Human gastric cancer cell lines SGC-7901 and MKN-45 were incubated with sub-cytotoxic concentrations of MJ as indicated. A and B, western blot and real-time quantitative RT-PCR indicated that administration of sub-cytotoxic (0.1 and 0.2 mM) MJ to SGC-7901 and MKN-45 cells for 24 hrs resulted in a decrease in the expression of VEGF, when compared to that of solvent-treated (mock) cells. C and D, western blot and real-time quantitative RT-PCR indicated that administration of 0.2 mM MJ to SGC-7901 and MKN-45 cells for 6, 12, and 24 hrs, resulted in the decrease of VEGF expression in a time-dependent manner, than that of mock cells. E and F, 72 hrs post-transfection of MMP-14 expression vector into SGC-7901 and MKN-45 cells, western blot and real-time quantitative RT-PCR indicated the over-expressed MMP-14 and VEGF than that of empty vector-transfected (mock) cells. G and H, 24 hrs post-transfection of si-MMP14 (100 nmol/L) into SGC-7901 and MKN-45 cells, western blot and real-time quantitative RT-PCR indicated the down-regulated MMP-14 and VEGF than those transfected with scramble siRNA (si-Scb, 100 nmol/L). The symbols (* and △) indicate a significant decrease and a significant increase from mock or si-Scb, respectively.
Zheng et al. BMC Cancer 2013 13:74 doi:10.1186/1471-2407-13-74