Figure 1.

Proliferation gradient characterization during Capan-2 multicellular tumor spheroids growth. (A) Immunodetection of proliferative cells (Ki-67, green) on a frozen section of a small Capan-2 spheroid (300 μm in diameter, left) or a large Capan-2 spheroid (500 μm in diameter, right). Nuclei are stained with DAPI (blue). Scale bar: 50 μm. (B) Visualization of proliferative cells after 24 h of EdU incorporation (red) on a frozen section of a small Capan-2 spheroid or a large spheroid. Nuclei are stained with DAPI (blue). Scale bar: 50 μm. (C) Graphic representation of the percentage of proliferative cells after 24 h of EdU incorporation related to the distance to spheroid surface within Capan-2 MCTS measuring 500 μm +/− 20 μm in diameter. Data correspond to the mean +/− SEM of percentage of EdU positive cells from 10 sections similar to the one shown in (B) from 4 different spheroids. (D) Visualization of the hypoxia by pimonidazole detection (green) on frozen sections from a small spheroid or a large spheroid. Nuclei are stained using DAPI (blue). Scale bar: 50 μm.

Laurent et al. BMC Cancer 2013 13:73   doi:10.1186/1471-2407-13-73
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